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KMID : 0811720060100000333
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Korean Journal of Physiology & Pharmacology
2006 Volume.10 No. 0 p.333 ~ p.0
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FRESHLY ISOLATED PACEMAKER CELL (CAJAL CELL) IN STOMACH OF GUINEA-PIG AND MOUSE
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Kim Young-Chul
Hikaru Suzuki
Kim Seung-Ryul
Youn Sei-Jin
Park Seon-Mee
Yun Hyo-Yung
Lee Sang-Jin
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Abstract
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Interstitial Cells of Cajal (ICC) were first described more than 100 years ago and until recently were believed to be confined to the gut from the esophagus to the anus, where they are now thought to be responsible for the generation and/or propagation of slow waves. Slow waves are generated within the tunica muscularis in many regions of the gastrointestinal (GI) tract. These events time the phasic contractions of the GI tract. To date, most researchers use cultured non single and tissue cultured network cells using specific murine tissue for this study. This protocol limits upper level of study for elucidating underlying mechanism of pacemaking response in human. Therefore, we tried to isolate and identify non cultured single ICC in adult animal. Voltage clamp technique and immunohistochemical study were used. Single ICC and smooth muscle cells were dissociated by enzyme treatment from murine and guinea-pig stomach. Single smooth muscle cell show spindle like morphology and have no branch. However, single ICC was discernible from smooth muscle cell for its morphology with many branches. Cells which have branches expressed c-Kit positive activity but smooth muscle cells do not expressed it under confocal microscope. In tissue level, we also found ICC network and intramuscular ICC which is sensitive to c-Kit in adult guinea-pig stomach. ICC also produced spontaneous pacemaking current under low Ca2+ buffering (0.1 mM EGTA) and K+-rich condition. However, it was not regular and robust. The peak amplitude of the spontaneous current was －329±107.2 (n=14). Under confocal Ca2+ imaging system, three types of ICCs were observed. From this study, we identified non-cultured and freshly isolated c-Kit positive single ICC cells which have traditional morphology and spontaneous beating in stomach of adult animal. It will promote the study of human GI motility using human Cajal cell soon.
Source: Korean J Physiol Pharmacol.2006 Oct;10(Suppl II):242
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KEYWORD
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Interstitial Cells of Cajal (ICC), GI tract, Guinea-pig, Mouse, Stomach
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